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(Plasmid #110185)


Item Catalog # Description Quantity Price (USD)
Plasmid 110185 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5715
  • Vector type
    Bacterial Expression, CRISPR ; i

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
    Synthetic; artificially synthesized, codon optimized gene from S. pyogenes
  • Insert Size (bp)
  • Mutation
    D10A, H840A
  • Promoter ermE*p

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pSET152-F (GATGTAGGAGGGCGTGGATATG)
  • 3′ sequencing primer pSET152-R (CCCAATACGCAAACCGCCTC)
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Insert Size (bp)
  • Promoter J23119

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSET-dCas9-actII-4-NT-S1 was a gift from Yinhua Lu (Addgene plasmid # 110185 ; ; RRID:Addgene_110185)
  • For your References section:

    CRISPR/dCas9-Mediated Multiplex Gene Repression in Streptomyces. Zhao Y, Li L, Zheng G, Jiang W, Deng Z, Wang Z, Lu Y. Biotechnol J. 2018 Jun 3. doi: 10.1002/biot.201800121. 10.1002/biot.201800121 PubMed 29862648