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Engineering orthogonal dual transcription factors for multi-input synthetic promoters.
Brodel AK, Jaramillo A, Isalan M
Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
80840M13KO7-ΔM13-ΔgeneIII-ΔgeneVIModified helper phage
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80843pLITMUS-rpoN-cI-J23106-geneIIIPhagemid (encodes M13 gene III and lambda cIopt).
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80852pLITMUS-rpoN-cIopt-J23106-geneIIIPhagemid (encodes M13 gene III and lambda cIopt).
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80858pJPC12-ΔM13-PRM-B0034-geneVIAccessory plasmid (encodes M13 gene VI)
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80859pJPC12-ΔM13-mCherry-PR/PRM-GFPReporter plasmid (mCherry and GFP)
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80860pLITMUS-rpoN-cI5C6A,P-J23106-geneIIIPhagemid (encodes evolved cI5C6A,P and M13 gene III)
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80861pLITMUS-rpoN-cI5G6G,P-J23106-geneIIIPhagemid (encodes evolved cI5G6G,P and M13 gene III)
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80862pLITMUS-rpoN-cI5G6T,P-J23106-geneIIIPhagemid (encodes evolved cI5G6T,P and M13 gene III)
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80863pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIIIPhagemid (encodes evolved cI4A5T6T,P and M13 gene III)
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80864pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIIIPhagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)
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80905pLITMUS-rpoN-cI5C6A-J23106-geneIIIPhagemid (encodes cI5C6A and M13 gene III)
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80906pLITMUS-rpoN-cI5G6G-J23106-geneIIIPhagemid (encodes cI5G6G and M13 gene III)
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80907pLITMUS-rpoN-cI5G6T-J23106-geneIIIPhagemid (encodes cI5G6T and M13 gene III)
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80908pLITMUS-rpoN-cI4A5T6T-J23106-geneIIIPhagemid (encodes cI4A5T6T and M13 gene III)
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80909pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIIIPhagemid (encodes cI4A5C6G7G and M13 gene III)
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80910pJPC12-ΔM13-mCherry-P/PM,5C6A-GFPReporter plasmid (mCherry and GFP)
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80911pJPC12-ΔM13-mCherry-P/PM,5G6G-GFPReporter plasmid (mCherry and GFP)
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80912pJPC12-ΔM13-mCherry-P/PM,5G6T-GFPReporter plasmid (mCherry and GFP)
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80913pJPC12-ΔM13-mCherry-P/PM,4A5T6T-GFPReporter plasmid (mCherry and GFP)
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80914pJPC12-ΔM13-mCherry-P/PM,4A5C6G7G-GFPReporter plasmid (mCherry and GFP)
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Antibodies from Article