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pLITMUS-rpoN-cI4A5T6T-J23106-geneIII
(Plasmid #80908)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80908 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLITMUS
  • Backbone manufacturer
    NEB
  • Total vector size (bp) 4924
  • Modifications to backbone
    M13 gene III expression cassette cI4A5T6T expression cassette
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    M13 gene III
  • Species
    M13 bacteriophage
  • Insert Size (bp)
    1275
  • Promoter BBa_J23106

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGTAGTTATCTACACGACG
  • 3′ sequencing primer TGCTTATACAATCTTCCTG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    cI4A5T6T
  • Species
    Synthetic
  • Insert Size (bp)
    714
  • Promoter rpoN

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BsaI (not destroyed)
  • 5′ sequencing primer gtc gat ttt tgt gat gct cg
  • 3′ sequencing primer ggg tta ttg tct cat gag cgg ata c
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC NGS identifies a SNP at nucleotide 1471 in the M13 ori. It is unknown what, if any, impact this may have on plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLITMUS-rpoN-cI4A5T6T-J23106-geneIII was a gift from Mark Isalan (Addgene plasmid # 80908 ; http://n2t.net/addgene:80908 ; RRID:Addgene_80908)
  • For your References section:

    Engineering orthogonal dual transcription factors for multi-input synthetic promoters. Brodel AK, Jaramillo A, Isalan M. Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858. 10.1038/ncomms13858 PubMed 27982027