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pJPC12-ΔM13-PRM-B0034-geneVI
(Plasmid #80858)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80858 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pJPC12
  • Total vector size (bp) 5300
  • Modifications to backbone
    Deletion of M13 packaging signal
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha ; TOP 10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    M13 gene VI
  • Species
    M13 bacteriophage
  • Insert Size (bp)
    339
  • Promoter Lambda Prm

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAA CGA CGG CCA GTG AGC
  • 3′ sequencing primer G ATA ACA ATT TCA CAC AGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pJPC12 vector was obtained from Peterson and Phillips (Peterson, J. and G.J. Phillips, New pSC101-derivative cloning vectors with elevated copy numbers. Plasmid, 2008. 59(3): p. 193-201)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJPC12-ΔM13-PRM-B0034-geneVI was a gift from Mark Isalan (Addgene plasmid # 80858 ; http://n2t.net/addgene:80858 ; RRID:Addgene_80858)
  • For your References section:

    Engineering orthogonal dual transcription factors for multi-input synthetic promoters. Brodel AK, Jaramillo A, Isalan M. Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858. 10.1038/ncomms13858 PubMed 27982027