pJPC12-ΔM13-PRM-B0034-geneVI
(Plasmid #80858)

• Purpose: Accessory plasmid (encodes M13 gene VI)
• Depositing Lab: Mark Isalan
• Publication: Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.

Backbone

• Vector backbone: pJPC12
• Total vector size (bp): 5300
• Modifications to backbone: Deletion of M13 packaging signal
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha ; TOP 10
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: M13 gene VI
• Species: M13 bacteriophage
• Insert Size (bp): 339
• Promoter: Lambda Prm

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAA CGA CGG CCA GTG AGC
• 3′ sequencing primer: G ATA ACA ATT TCA CAC AGG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pJPC12 vector was obtained from Peterson and Phillips (Peterson, J. and G.J. Phillips, New pSC101-derivative cloning vectors with elevated copy numbers. Plasmid, 2008. 59(3): p. 193-201)