163,703 results

1. pCAGGS puro human-GFP-RLIM-IRES-mCherry

   Plasmid

   #232243

   Purpose

   Mammalian expression of RLIM-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   RLIM-GFP-IRES-mCherry (RLIM Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

   Plasmid

   #232244

   Purpose

   Mammalian expression of N581K RLIM-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   N581K RLIM-GFP-IRES-mCherry (RLIM Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   N581K

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS puro GFP-REX1[degron]-IRES-mCherry

   Plasmid

   #232240

   Purpose

   Mammalian expression of REX1[degron]-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   REX1 (aa 70-85)

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB1163

   Plasmid

   #210032

   Purpose

   Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas11-P2A-Cas6-T2A-Cas3
   rtta-T2A-blast
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. CA-0192 CMVTO-Renin(mouse)-ECL-CD4-tevs-RXR6.2

   Plasmid

   #231204

   Purpose

   ER-retained mouse renin

   Depositor

   Xiaojing Gao

   Article

   Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

   Insert

   Renin(mouse)-ECL-CD4-tevs-RXR6.2 (Ren1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. CA-0193 CMVTO-Renin(H2M)-ECL-CD4-tevs-RXR6.2

   Plasmid

   #231203

   Purpose

   ER-retained H2M renin

   Depositor

   Xiaojing Gao

   Article

   Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

   Insert

   Renin(H2M)-ECL-CD4-tevs-RXR6.2 (REN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L147I, R148H, I203V, L290V

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-30nmEML-mRFP

   Plasmid

   #231182

   Purpose

   Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 30 nm from a lentiviral vector

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   30nmEML-mRFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV syn Barcode1

   Plasmid

   #229062

   Purpose

   Expression mapping

   Depositor

   Scott Sternson

   Insert

   Syn Barcode1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCKC775

   Plasmid

   #226626

   Purpose

   piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Inserts

   7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT S. pyogenes, Human)
   EF1a promoter, sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   7xTCF-LEF and EF1a

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCKC764

    Plasmid

    #226625

    Purpose

    piggyBAC pCAG-hCas9-T2A-Unbiased3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Chicken, S. pyogenes, Human)
    EF1a promoter, blasticidin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCKC776

    Plasmid

    #226627

    Purpose

    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, mCherry
    

    Use

    Synthetic Biology

    Tags

    oxygen dependent degron (ODD)

    Expression

    Mammalian

    Mutation

    Promoter

    4xHRE_YB TATA and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUdOm6.1

    Plasmid

    #210286

    Purpose

    Empty backbone with trimethoprim resistance for high expression in mammalian cells

    Depositor

    Corrie daCosta

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn-pDisplay-SNAP-T2A-EGFP

    Plasmid

    #223503

    Purpose

    Co-expression of SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker

    Depositor

    James Frank

    Insert

    SNAP-tag and EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)

    Plasmid

    #215759

    Purpose

    HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

    Insert

    CD247-truncCD19CAR-GSG (CD19, CD247 Human)
    

    Use

    Tags

    IgG1 CH3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCM4.4

    Plasmid

    #217648

    Purpose

    an ermE*p-cas9-driven expression, as an alternative plasmid

    Depositor

    Huimin Zhao

    Article

    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

    Insert

    Codon Optimized SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AcGFP-PMS2

    Plasmid

    #215124

    Purpose

    Expresses AcGFP-PMS2 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PMS2 (PMS2 Human)
    

    Use

    Retroviral

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving

    Plasmid

    #204810

    Purpose

    Bacterial expresison of Enterovirus A71 2A protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    A71 2A protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. TFORF3312

    Plasmid

    #144788

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELK4 (ELK4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF1030

    Plasmid

    #143461

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RARG (RARG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-Zyxin-D (aa160-340)-EGFP

    Plasmid

    #187696

    Purpose

    Visualization of Zyxin

    Depositor

    Mary Beckerle

    Article

    Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

    Insert

    Zyxin (Zyx Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa160-340

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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