167,948 results

1. KJ740(DE3)

   Bacterial Strain

   #179486

   Purpose

   KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Bacterial Resistance

   Tetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)

   Species

   Escherichia coli

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

2. NPM1_mNeonGreen_Donor

   Plasmid

   #178093

   Purpose

   HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   NPM1-mNeonGreen HDR donor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. BLBP-mCherry

   Plasmid

   #63721

   Purpose

   Expressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

   Depositor

   Bart De Strooper

   Article

   Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

   Insert

   BLBP (Fabp7 Mouse)
   

   Expression

   Mammalian

   Mutation

   1700 bp upstream of BLBP gene cloned in CAG-mCher…

   Promoter

   1700bp upstream of BLBP gene

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBI-EGFP-MnSOD

   Plasmid

   #16612

   Depositor

   Bert Vogelstein

   Article

   Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

   Insert

   MnSOD (SOD2 Human)
   

   Expression

   Mammalian

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1-rtTA-PGK-BSD

   Plasmid

   #192885

   Purpose

   expression of rtTA

   Depositor

   Kazuhiro Oka

   Insert

   rtTA
   

   Use

   Lentiviral

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0095

   Plasmid

   #142145

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GW1-Mito-pHRed

   Plasmid

   #31474

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   Cox8-tagged pHRed
   

   Tags

   4xCox8

   Expression

   Mammalian

   Available Since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. Circular-oc-EMCV-GFP

   Plasmid

   #226261

   Purpose

   Production of in vitro circularizable RNA via IVT

   Depositor

   Prashant Mali

   Article

   Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

   Insert

   EGFP
   

   Use

   In vitro transcription

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.4_7C11-1 light chain

   Plasmid

   #198252

   Purpose

   recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)
   

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.4_7C11-1 heavy chain

    Plasmid

    #198251

    Purpose

    recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)
    

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-pro-Il1b-C-Flag

    Plasmid

    #75131

    Purpose

    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    interleukin 1 beta (Il1b Mouse)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFPc1 IP3R3 WT

    Plasmid

    #236453

    Purpose

    transient overexpression of IP3R3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

    Insert

    IP3R3 (Itpr3 Rat)
    

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKCyR8

    Plasmid

    #149494

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CymR(AM)-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PCymRC

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Human Parkin (1-465)

    Plasmid

    #110758

    Purpose

    Bacterial expression of human Parkin

    Depositor

    David Komander

    Article

    Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

    Insert

    PRKN (PRKN Human)
    

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRC-APEX2

    Plasmid

    #72558

    Purpose

    Expression of cytosolic APEX2 in bacteria

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A134P relative to APEX

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriExRhoA2G

    Plasmid

    #40176

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

    Plasmid Kit

    #1000000221

    Purpose

    A positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.

    Depositor

    Eric Cantor

    Available Since

    June 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pET MBP mCherry LIC cloning vector (MBP-mCherry)

    Plasmid

    #29747

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Available Since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-ATG5

    Plasmid

    #13095

    Depositor

    Roberta Gottlieb

    Article

    Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

    Insert

    ATG5 (Atg5 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ucp1-Cre-miR122

    Plasmid

    #228408

    Purpose

    Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

    Depositor

    Joerg Heeren

    Article

    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

    Inserts

    cre recombinase with SV40 NLS
    Cre recombinase with SV40 NLS
    

    Use

    AAV

    Promoter

    rat Ucp1 enhancer-promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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