165,637 results

1. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138131

   Purpose

   Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-rabbit-IgG(Fc)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FNLCR CRISPRa Cell Surface

   Pooled Library

   #207471

   Purpose

   CRISPR-based activation library with guide RNAs targeting all cell surface proteins.

   Depositor

   Raj Chari

   Article

   Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

3. pET15b_Bst-LF

   Plasmid

   #153313

   Purpose

   Bacterial expression of the Bst Large Fragment

   Depositor

   Drew Endy, Philippa Marrack

   Insert

   Bst Large Fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74009

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5x UAS
   Cas9
   T2A
   GFP
   U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-mPIAS1

   Plasmid

   #15206

   Depositor

   Ke Shuai

   Article

   Liu et al Proc Natl Acad Sci U S A. 1998 Sep 1. 95

   Insert

   PIAS1 (Pias1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. CamKII-LGI1-pHmScarlet

   Plasmid

   #185538

   Purpose

   Expresses LGI1-pHmScarlet under the CamKII promoter

   Depositor

   Jaime de Juan-Sanz

   Article

   Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

   Insert

   LGI1 (Lgi1 Rat)
   

   Use

   Lentiviral

   Tags

   pHmScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0295

   Plasmid

   #142591

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MEF2A (MEF2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB80-hKif5b(1-560)rigor-2xmNeongreen

   Plasmid

   #174649

   Purpose

   Live-cell marker for stable microtubules

   Depositor

   Lukas Kapitein

   Article

   Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

   Insert

   hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
   

   Use

   Tags

   mNeongreen-mNeongreen

   Expression

   Mammalian

   Mutation

   rigor mutation G234A

   Promoter

   Chicken beta-actin

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. P362-FBP_12

   Plasmid

   #139703

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep

    Plasmid

    #173899

    Purpose

    The pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThr

    Depositor

    Jason W Chin

    Article

    Zhang et al Nat Methods. 2017 Jul;14(7):729-736. doi

    Inserts

    ThrRS(v1.0)
    tRNA(v2.0)CUA
    pduX
    EF-Sep
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (AAV Retrograde)

    Viral Prep

    #162382-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (#162382). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of the ultrafast calcium sensor GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    CAG

    Tags

    None

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-EF1a-CCreV

    Plasmid

    #140132

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    CCreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1a-NCreV

    Plasmid

    #140131

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    NCreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. ER-J-TAG (pGS730)

    Plasmid

    #160038

    Purpose

    pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Insert

    3xHA-sfGFP-JPH-TMD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG413GPD-ccdB

    Plasmid

    #14142

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TetO-U2B''-fSNAPc

    Plasmid

    #106988

    Purpose

    open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    UB2" (SNRPB2 Human)
    

    Use

    Tags

    fSNAP

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICH41744

    Plasmid

    #48017

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. Perox-RA

    Plasmid

    #209869

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRUPATH Triple GaoB

    Plasmid

    #196052

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST14-SpyCatcher002

    Plasmid

    #102827

    Purpose

    Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyCatcher002
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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