163,703 results

1. AP-Ykt6

   Plasmid

   #167997

   Purpose

   positive selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   Ykt6(L161-L177)-T7PAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_GRB2_SH3

   Plasmid

   #110015

   Purpose

   Protein expression and purification of GRB2_SH3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GRB2_SH3 (GRB2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMR-A-5

   Plasmid

   #138519

   Purpose

   P3c

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   T7-RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGST-DHFR Mm

   Plasmid

   #83073

   Purpose

   Expresses the full length DHFR Mm in E.coli

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   2x DHFR (Dhfr Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKM461

   Plasmid

   #108320

   Purpose

   For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Inserts

   Che9C RecT
   Bxb1 Integrase
   Tet Repressor
   SacRB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. B-95.ΔA

   Bacterial Strain

   #197933

   Purpose

   BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

   Bacterial Resistance

   None

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

7. pUK21

   Plasmid

   #49788

   Purpose

   E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1991 Apr;100:189-94.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lacZ

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. FPR2-Tango

   Plasmid

   #66284

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   FPR2 (FPR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pet28a_Human_H4

   Plasmid

   #42633

   Depositor

   Joe Landry

   Insert

   Human H4 (H4C1 Human)
   

   Use

   Tags

   Untagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 25, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCAG-VSVM

    Plasmid

    #64086

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene

    Depositor

    Ian Wickersham

    Insert

    VSV matrix protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIV-H2B-eGFP

    Plasmid

    #91776

    Purpose

    Expresses H2B protein tagged with eGFP

    Depositor

    Maria Pia Cosma

    Article

    Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

    Insert

    Human Histone H2B / eGFP (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSR657

    Plasmid

    #65214

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-2 cap
    

    Use

    AAV

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10 and polyhedrin

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDY0973-RADARS iCaspase

    Plasmid

    #182539

    Purpose

    Express RADARS iCaspase that conditionally turn on iCaspase in the presence of target RNA

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Insert

    iCaspase9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK19pA-MN

    Plasmid

    #86973

    Purpose

    ChIC; genomic mapping of chromatin proteins

    Depositor

    Ulrich Laemmli

    Article

    Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

    Insert

    protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.EF1a.ChR2-YFP.WPRE.hGH

    Plasmid

    #100056

    Purpose

    AAV expression of ChR2 fused to YFP driven byEF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Insert

    ChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7C-HSP90AB1

    Plasmid

    #177659

    Purpose

    Recombinant protein synthesis of human HSP90AB1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSP90AB1 (HSP90AB1 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE 80L Kan T7WT

    Plasmid

    #102790

    Purpose

    Expresses T7 RNA polymerase

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pFRT/FLAG/HA-DEST PUM2

    Plasmid

    #40292

    Depositor

    Thomas Tuschl

    Article

    Hafner et al Cell. 2010 Apr 2;141(1):129-41.

    Insert

    Pumilio homolog 2 (PUM2 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Lyn-ICUE3

    Plasmid

    #61623

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    Lyn-ICUE3 (RAPGEF3 Human)
    

    Use

    Tags

    ECFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.3-SARS2-B.1.617.2

    Plasmid

    #172320

    Purpose

    expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.2 strain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

    Promoter

    CMV-F

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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