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pIM1773-PRHIII-sfGFP-pNW33N
(Plasmid #52217)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52217 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNW33N
  • Backbone manufacturer
    Bacillus Genetic Stock Center
  • Backbone size w/o insert (bp) 4217
  • Total vector size (bp) 5062
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    InvaF'
  • Growth instructions
    This plasmid is generally slow growing in E. coli. Growth in Geobacillus is generally on par with untransformed strains.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRHIII-sfGFP
  • Species
    Synthetic
  • Promoter PRHIII

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer M13-Rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Plasmid is designed for use in Geobacillus stearothermophilus NUB3621 (as well as E. coli.) Construct is described in the article linked at the bottom of this page. This plasmid is a companion to pIM1708-PsurP-surT-agaN-pNW33N which was previously submitted. As with pIM1708, despite the fact that your plasmid map displays an ampicillin resistance gene, the correct antibiotic resistance gene is a chloramphenicol acetyltransferase. We do not know if the ampicillin resistance gene is functional.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIM1773-PRHIII-sfGFP-pNW33N was a gift from Ichiro Matsumura (Addgene plasmid # 52217 ; http://n2t.net/addgene:52217 ; RRID:Addgene_52217)
  • For your References section:

    Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering. Blanchard K, Robic S, Matsumura I. Appl Microbiol Biotechnol. 2014 May 2. 10.1007/s00253-014-5746-z PubMed 24788326