PurposeConstitutive sfGFP expression plasmid for use in Geobacillus and E. coli
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52217||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerBacillus Genetic Stock Center
- Backbone size w/o insert (bp) 4217
- Total vector size (bp) 5062
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsThis plasmid is generally slow growing in E. coli. Growth in Geobacillus is generally on par with untransformed strains.
- Promoter PRHIII
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Terms and Licenses
Plasmid is designed for use in Geobacillus stearothermophilus NUB3621 (as well as E. coli.) Construct is described in the article linked at the bottom of this page. This plasmid is a companion to pIM1708-PsurP-surT-agaN-pNW33N which was previously submitted. As with pIM1708, despite the fact that your plasmid map displays an ampicillin resistance gene, the correct antibiotic resistance gene is a chloramphenicol acetyltransferase. We do not know if the ampicillin resistance gene is functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIM1773-PRHIII-sfGFP-pNW33N was a gift from Ichiro Matsumura (Addgene plasmid # 52217 ; http://n2t.net/addgene:52217 ; RRID:Addgene_52217)
For your References section:Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering. Blanchard K, Robic S, Matsumura I. Appl Microbiol Biotechnol. 2014 May 2. 10.1007/s00253-014-5746-z PubMed 24788326