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pIM1708-surT-PsurP-agaN-pNW33N
(Plasmid #44009)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44009 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNW33N
  • Backbone manufacturer
    Bacillus Genetic Stock Center
  • Backbone size w/o insert (bp) 4174
  • Total vector size (bp) 7680
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    INVaF'
  • Growth instructions
    May require up to 24 hours of growth for sufficient colonies when plated (and for preps)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    surT-PsurP-agaN
  • Alt name
    alpha galactosidase
  • Alt name
    Geobacillus stearothermophilus transcription antiterminator
  • Species
    Geobacillus stearothermophilus NUB3621
  • Insert Size (bp)
    3506
  • Mutation
    Deleted EcoRI site (synonymous change, C to T at position 967) in surT region
  • GenBank ID
    AF130985.1 U34873.1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

IMPORTANT NOTE: Even though there is a partial AmpR gene noted on the map, this plasmid is NOT known to confer Amp resistance. The CAT gene is the first ORF (270-920) and the Matsumura lab has only used Chloramphenicol for selection. The backbone, pNW33N, was not engineered by the depositing lab and the sequence they present here is theoretical. Though there are discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor, the plasmid should function as described.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIM1708-surT-PsurP-agaN-pNW33N was a gift from Ichiro Matsumura (Addgene plasmid # 44009 ; http://n2t.net/addgene:44009 ; RRID:Addgene_44009)
  • For your References section:

    Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering. Blanchard K, Robic S, Matsumura I. Appl Microbiol Biotechnol. 2014 May 2. 10.1007/s00253-014-5746-z PubMed 24788326