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pSEVA3b67Rb
(Plasmid #58311)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58311 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    In E. coli, use chloramphenicol at 6 µg/mL (gene expression of cat not very strong). In B. subtilis, the same concentration is sufficient for selection.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRFP1
  • Species
    Synthetic

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more info on shuttle vector backbone, see entry for pSEVA3b61.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVA3b67Rb was a gift from Tom Ellis (Addgene plasmid # 58311 ; http://n2t.net/addgene:58311 ; RRID:Addgene_58311)
  • For your References section:

    GeneGuard: a Modular Plasmid System Designed for Biosafety. Wright O, Delmans M, Stan GB, Ellis T. ACS Synth Biol. 2014 May 13. 10.1021/sb500234s PubMed 24847673