|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71739||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4719
- Total vector size (bp) 5650
Vector typeBacterial Expression, Synthetic Biology ; Shuttle Vector
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Copy numberHigh Copy
Alt namesuper folder Green Fluorescent Protein
Insert Size (bp)720
- Promoter pRplsWT
- Cloning method Gibson Cloning
- 5′ sequencing primer -
- 3′ sequencing primer AGCGGATAACAATTTCACACAGGA (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe Leak lab, (Department of Biology & Biochemistry, University of Bath). Part of these plasmids are from pUCG18.
Terms and Licenses
- Not Available to Industry
Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pG1AK-sfGFP was a gift from Tom Ellis (Addgene plasmid # 71739 ; http://n2t.net/addgene:71739 ; RRID:Addgene_71739)
For your References section:The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. Reeve B, Martinez-Klimova E, de Jonghe J, Leak DJ, Ellis T. ACS Synth Biol. 2016 Jul 1. 10.1021/acssynbio.5b00298 PubMed 27332993