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Christopher A Walsh Lab Plasmids


The Christopher A Walsh Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Walsh Lab unpublished plasmids (unpublished)
Evolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Bae BI, Tietjen I, Atabay KD, Evrony GD, Johnson MB, Asare E, Wang PP, Murayama AY, Im K, Lisgo SN, Overman L, Sestan N, Chang BS, Barkovich AJ, Grant PE, Topcu M, Politsky J, Okano H, Piao X, Walsh CA Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
32530pCMV-HA (New MCS)Mammalian Expression Walsh Lab unpublished plasmids (unpublished) pCMV-HA
  • Tag / Fusion Protein
    • HA (N terminal on backbone)
  • Add to Cart
    52296pCAG-DN-RFXDominant negative RFX (Mus musculus)Mammalian ExpressionEvolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392. Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag. pCAGEN Add to Cart
    52297pCAG-hGPR56-IRES-GFPHuman GPR56 coding sequence (Homo sapiens)Mammalian ExpressionEvolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392. Expresses human GPR56 and GFP in mammalian cells. pCAGIG ADGRG1 BFPP, BPPR, GPR56, TM7LN4, TM7XN1 Add to Cart
    52298pGL3E-hGPR56 e1m promoter normalHuman GPR56 e1m promoter (Homo sapiens)LuciferaseEvolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392. Reporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E. pGL3E Add to Cart
    52299pGL3E-hGPR56 e1m promoter perisylvian polymicrogyriaHuman GPR56 e1m promoter (Homo sapiens)LuciferaseEvolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392. Reporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E. pGL3E Add to Cart