163,682 results

1. pAAV-CAG-DIO(ChRger2-TS-YFP)

   Plasmid

   #127239

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger2-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SIRT1 Flag

   Plasmid

   #13812

   Purpose

   Mammalian expression of human SIRT1 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT1 (SIRT1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   N-terminus truncated

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b_PstS_1

   Plasmid

   #78198

   Purpose

   Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A197C (please see depositors comment below)

   Promoter

   T7

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUPD2-HR1b-GFP-L (B3-B4)

   Plasmid

   #211853

   Purpose

   E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEC2483 (pSpy1C)

   Plasmid

   #220248

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX304 Luciferase-V5 blast

   Plasmid

   #98580

   Purpose

   Lentiviral vector for constitutive expression of Luciferase with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG CMV N-HA Puro

   Plasmid

   #237879

   Purpose

   Empty mammalian expression plasmid for cloning N-terminally HA-tagged proteins

   Depositor

   Addgene Research Program, Meghan Rego

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pUC19_msfGFP-EWSR1-repair-tempate

   Plasmid

   #237682

   Purpose

   For knock-in msfGFP to EWSR1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX458_mCherry-EWSR1-gRNA

   Plasmid

   #237683

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to EWSR1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EWSR1 gRNA (EWSR1 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

    Plasmid

    #236246

    Purpose

    Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    U6 and human Synapsin1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTM-NiV-L

    Plasmid

    #234657

    Purpose

    Expresses L gene of NiV Bangladesh strain

    Depositor

    Carolina Lopez

    Article

    Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

    Insert

    NiV L gene (L Nipah virus)
    

    Use

    In cell lines expressing bacteriophage t7 rna pol…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTM-NiV-P

    Plasmid

    #234656

    Purpose

    Expresses P gene of NiV Bangladesh strain

    Depositor

    Carolina Lopez

    Article

    Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

    Insert

    NiV P gene (Nvgp2 Nipah virus)
    

    Use

    In cell lines expressing bacteriophage t7 rna pol…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTM-NiV-N

    Plasmid

    #234655

    Purpose

    Expresses N gene of NiV Bangladesh strain

    Depositor

    Carolina Lopez

    Article

    Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

    Insert

    NiV N gene (N Nipah virus)
    

    Use

    In cell lines expressing bacteriophage t7 rna pol…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAX1_HS_gpN:SnoopTag

    Plasmid

    #225195

    Purpose

    Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SnoopTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL1180-NiV L(2k)-mCherry/eGFP

    Plasmid

    #234654

    Purpose

    Minigenome plasmid of NiV Bangladesh strain

    Depositor

    Carolina Lopez

    Article

    Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

    Inserts

    part L gene
    mCherry
    eGFP
    

    Use

    In cell lines expressing bacteriophage t7 rna pol…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease

    Plasmid

    #228636

    Purpose

    Bacterial expression of the A71 3C protease (isoform B) used in crystallisation

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Enterovirus A71 3C protease isoform B
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCAGGS puro human-GFP-RLIM-IRES-mCherry

    Plasmid

    #232243

    Purpose

    Mammalian expression of RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

    Plasmid

    #232244

    Purpose

    Mammalian expression of N581K RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    N581K RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    N581K

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS puro GFP-REX1[degron]-IRES-mCherry

    Plasmid

    #232240

    Purpose

    Mammalian expression of REX1[degron]-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    REX1 (aa 70-85)

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB1163

    Plasmid

    #210032

    Purpose

    Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas11-P2A-Cas6-T2A-Cas3
    rtta-T2A-blast
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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