167,948 results

1. pETNb-cALFA

   Plasmid

   #159989

   Purpose

   For the protein expression with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_BiCHATe27_ChR2_mCherry (AAV PHP.eB)

   Viral Prep

   #213830-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV_BiCHATe27_ChR2_mCherry (#213830). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the cholinergic neuron-targeting enhancer E27. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Tags

   mCherry

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

3. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

   Plasmid

   #132404

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Dcp1b

   Plasmid

   #153976

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1b

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA recapping enzybe 1b (DCP1B Human)
   

   Tags

   pAcGFP-C1

   Expression

   Mammalian

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. STICR_Index3

   Pooled Library

   #186335

   Purpose

   High Complexity Lentiviral Barcode Library

   Depositor

   Tomasz Nowakowski

   Article

   Delgado et al Nature. 2021 Dec 15. pii: 10.1038/s41586

   Expression

   Mammalian

   Species

   Synthetic

   Use

   Lentiviral

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

7. pCDB179-TurboPETase

   Plasmid

   #218693

   Purpose

   Encodes codon optimized TurboPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   TurboPETase
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAB167

   Plasmid

   #101672

   Purpose

   Opto-T7RNAP*(563), equal expr.

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   Opto-T7RNAP*(563), equal expr.
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRF-TetR

   Plasmid

   #49374

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   TetR repressor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-Wnt7B

    Plasmid

    #35915

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt7B (Wnt7b Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. SMARCA2-GFP

    Plasmid

    #65390

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SMARCA2 (SMARCA2 Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MAC-DPP4

    Plasmid

    #172416

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Dipeptidyl peptidase 4 (DPP4 Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pInt-ts

    Plasmid

    #66076

    Purpose

    The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

    Insert

    lambda integrase
    

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. gRNA_Cloning Vector BbsI

    Plasmid

    #128433

    Purpose

    Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing

    Depositor

    Izuho Hatada

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBR_attB(bxb)_lox

    Plasmid

    #183762

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. HCA2-Tango

    Plasmid

    #66396

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HCA2 (HCAR2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. siRNA-resistant SigmaR1-mNeonGreen R119A F191A

    Plasmid

    #226574

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    R119A, F191A

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TLCV2-LoxP

    Plasmid

    #127098

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system.

    Depositor

    Adam Karpf

    Insert

    Cas9-2A-eGFP
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET GFP LIC cloning vector (u-msfGFP)

    Plasmid

    #29772

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    SiriusGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR SFFVp Phy-mCherry-CAAX

    Plasmid

    #50839

    Purpose

    Optogenetic PI3K activation system; membrane component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

    Insert

    PhyB 1-908
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    mCherry

    Promoter

    SFFV

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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