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Random Insertion of mCherry Into VP3 Domain of Adeno-associated Virus Yields Fluorescent Capsids With no Loss of Infectivity.
Judd J, Wei F, Nguyen PQ, Tartaglia LJ, Agbandje-McKenna M, Silberg JJ, Suh J
Mol Ther Nucleic Acids. 2012 Nov 13;1:e54. doi: 10.1038/mtna.2012.46.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
65723pAAV2_VP1_RaPID_r1c3Produce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 only
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65724pRC_RR_VP1_r1c3Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR
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65725pRC_RR_VP2/3wt VP2/VP3 only to participate in capsid assembly; no ITR's
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