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Cas9/pTREX-n
(Plasmid #68708)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68708 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 68708-D50 50 μg of DNA in Tris buffer $495
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DNA 68708-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTREX-n
  • Backbone manufacturer
    Vazquez and Levin., 1999
  • Backbone size w/o insert (bp) 6227
  • Total vector size (bp) 11173
  • Modifications to backbone
    Cloning of gene Cas9-HA-2xNLS-GFP mutated (C4239T) into pTREX-n vector
  • Vector type
    CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
  • Alt name
    Cas9-GFP
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    4975
  • Mutation
    C4239T mutation that eliminates a BamHI restriction site on the Cas9-GFP ORF.
  • Tag / Fusion Protein
    • Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene was cloned by Dr. Noelia Lander in my laboratory. The sequence was obtained from vector pMJ920 (Addgene), kindly provided by Dr. Vasant Muralidharan (University of Georgia, Athens, GA).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cas9/pTREX-n was a gift from Roberto Docampo (Addgene plasmid # 68708 ; http://n2t.net/addgene:68708 ; RRID:Addgene_68708)

  • For your References section:

    CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment. Lander N, Li ZH, Niyogi S, Docampo R. MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15. 10.1128/mBio.01012-15 PubMed 26199333
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