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Cas9/pTREX-n
(Plasmid #68708)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68708 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTREX-n
  • Backbone manufacturer
    Vazquez and Levin., 1999
  • Backbone size w/o insert (bp) 6227
  • Total vector size (bp) 11173
  • Modifications to backbone
    Cloning of gene Cas9-HA-2xNLS-GFP mutated (C4239T) into pTREX-n vector
  • Vector type
    CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
  • Alt name
    Cas9-GFP
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    4975
  • Mutation
    C4239T mutation that eliminates a BamHI restriction site on the Cas9-GFP ORF.
  • Tag / Fusion Protein
    • Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene was cloned by Dr. Noelia Lander in my laboratory. The sequence was obtained from vector pMJ920 (Addgene), kindly provided by Dr. Vasant Muralidharan (University of Georgia, Athens, GA).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cas9/pTREX-n was a gift from Roberto Docampo (Addgene plasmid # 68708 ; http://n2t.net/addgene:68708 ; RRID:Addgene_68708)
  • For your References section:

    CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment. Lander N, Li ZH, Niyogi S, Docampo R. MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15. 10.1128/mBio.01012-15 PubMed 26199333