pUC_sgRNA
(Plasmid #68710)

• Purpose: (Empty Backbone) Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
• Depositing Lab: Roberto Docampo
• Publication: Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15.

Backbone

• Vector backbone: pUCAmp
• Backbone manufacturer: Blue Heron
• Backbone size (bp): 3150
• Modifications to backbone: tracrRNA sequence (82 bp) was custom-made synthesized by Blue Heron and inserted into the cloning site of pUCAmp vector
• Vector type: CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13_forward20_primer and M13_pUC_fwd_primer
• 3′ sequencing primer: M13_reverse_primer and M13_pUC_rev_primer

Resource Information

• Supplemental Documents:
  • Blue Heron pUCAmp vector map
• A portion of this plasmid was derived from a plasmid made by: We purchased this custom-made plasmid from Blue Heron Biotech, LLC (Bothell, WA 98021 USA)
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry