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pENTR-OsMIR390-B/c
(Plasmid #61468)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61468 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pENTR
  • Backbone size w/o insert (bp) 2592
  • Total vector size (bp) 4122
  • Vector type
    GATEWAY entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OsMIR390-B/c
  • Species
    Oryza sativa
  • Insert Size (bp)
    1530
  • Mutation
    O. sativa MIR390 precursor sequence including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites. The BsaI site in the ccdB gene was mutated to block the site.

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-OsMIR390-B/c was a gift from James Carrington (Addgene plasmid # 61468)
  • For your References section:

    Highly Specific Gene Silencing in a Monocot Species by Artificial MicroRNAs Derived From Chimeric MIRNA Precursors. Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, Carrington JC. Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835. 10.1111/tpj.12835 PubMed 25809382