Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #61467)


Item Catalog # Description Quantity Price (USD)
Plasmid 61467 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 10145
  • Total vector size (bp) 11675
  • Modifications to backbone
    A BsaI site of the backbone was mutated to block the site.
  • Vector type
    Plant Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Oryza sativa
  • Insert Size (bp)
  • Mutation
    O. sativa MIR390 precursor sequence including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites. The BsaI site in the ccdB gene was mutated to block the site.
  • Promoter 2x35S

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDC32B-OsMIR390-B/c was a gift from James Carrington (Addgene plasmid # 61467 ; ; RRID:Addgene_61467)
  • For your References section:

    Highly Specific Gene Silencing in a Monocot Species by Artificial MicroRNAs Derived From Chimeric MIRNA Precursors. Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, Carrington JC. Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835. 10.1111/tpj.12835 PubMed 25809382