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DNase H Activity of Neisseria meningitidis Cas9.
Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ
Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
71474pMCSG7-Wt-NmeCas9bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV site
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71475pMCSG7-dNmeCas9bacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV site
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71476pMCSG7-D16A-NmeCas9bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site
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71477pMCSG7-H588A-NmeCas9bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site
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