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pMCSG7-D16A-NmeCas9
(Plasmid #71476)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71476 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMCSG7
  • Backbone manufacturer
    MCSG
  • Backbone size w/o insert (bp) 5286
  • Total vector size (bp) 8541
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pMCSG7-D16A-NmeCas9
  • Species
    Neisseria meningitidis
  • Insert Size (bp)
    3321
  • Mutation
    D16A
  • Tag / Fusion Protein
    • 6His (N terminal on insert)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCSG7-D16A-NmeCas9 was a gift from Erik Sontheimer (Addgene plasmid # 71476 ; http://n2t.net/addgene:71476 ; RRID:Addgene_71476)
  • For your References section:

    DNase H Activity of Neisseria meningitidis Cas9. Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ. Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020. 10.1016/j.molcel.2015.09.020 PubMed 26474066