|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||87700||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)3349
/ Fusion Protein
- 10xHis-MBP-TEV (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer GATGAAGCCCTGAAAGACGCGCAG
- 3′ sequencing primer CTA GTT ATT GCT CAG CGG T (Common Sequencing Primers)
Please see http://biorxiv.org/content/early/2017/05/16/138867 for the preprint publication describing this construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-MBP-Geo_st was a gift from Jennifer Doudna (Addgene plasmid # 87700 ; http://n2t.net/addgene:87700 ; RRID:Addgene_87700)