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(Plasmid #71477)


Item Catalog # Description Quantity Price (USD)
Plasmid 71477 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5286
  • Total vector size (bp) 8541
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    Neisseria meningitidis
  • Insert Size (bp)
  • Mutation
  • Promoter T7
  • Tag / Fusion Protein
    • 6His (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TAC TTC CAA TCC AAT GCC ATG GCT GCC TTC AAA CC
  • 3′ sequencing primer TTA TCC ACT TCC AAT GTT TTA ACG GAC AGG CGG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCSG7-H588A-NmeCas9 was a gift from Erik Sontheimer (Addgene plasmid # 71477 ; ; RRID:Addgene_71477)
  • For your References section:

    DNase H Activity of Neisseria meningitidis Cas9. Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ. Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020. 10.1016/j.molcel.2015.09.020 PubMed 26474066