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High-throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae.
Liu X, Gallay C, Kjos M, Domenech A, Slager J, van Kessel SP, Knoops K, Sorg RA, Zhang JR, Veening JW
Mol Syst Biol. 2017 May 10;13(5):931. doi: 10.15252/msb.20167449.
PubMed

Plasmids from Article

ID Plasmid Purpose  
85588pJWV102-PL-dCas9Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp
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85589pPEPY-PF6-lacIWork as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae
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85590pPEPX-P3-sgRNAlucIntegrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for
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