An open library of human kinase domain constructs for automated bacterial expression.
Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081. PubMed Article
Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
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| 79680 | MK14_HUMAN_D0 | This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79681 | VRK3_HUMAN_D0 | This plasmid encodes the kinase domain of VRK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79682 | GAK_HUMAN_D0 | This plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79683 | CSK_HUMAN_D0 | This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79684 | VRK1_HUMAN_D0 | This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79685 | KC1G3_HUMAN_D0 | This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79686 | FES_HUMAN_D0 | This plasmid encodes the kinase domain of FES. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79687 | PMYT1_HUMAN_D0 | This plasmid encodes the kinase domain of PMYT1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79688 | MK03_HUMAN_D0 | This plasmid encodes the kinase domain of MK03. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79689 | STK3_HUMAN_D0 | This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79690 | DYR1A_HUMAN_D0 | This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79691 | KC1G1_HUMAN_D0 | This plasmid encodes the kinase domain of KC1G1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79692 | MK11_HUMAN_D0 | This plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79693 | MK13_HUMAN_D0 | This plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79694 | EPHB1_HUMAN_D0 | This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79695 | MK08_HUMAN_D0 | This plasmid encodes the kinase domain of MK08. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79696 | CDK16_HUMAN_D0 | This plasmid encodes the kinase domain of CDK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79697 | EPHB2_HUMAN_D0 | This plasmid encodes the kinase domain of EPHB2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79698 | PAK4_HUMAN_D0 | This plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79699 | CDKL1_HUMAN_D0 | This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79700 | SRC_HUMAN_D0 | This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79701 | STK16_HUMAN_D0 | This plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79702 | MAPK3_HUMAN_D0 | This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79703 | PAK6_HUMAN_D0 | This plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79704 | CSK22_HUMAN_D0 | This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79705 | MERTK_HUMAN_D0 | This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79706 | PAK7_HUMAN_D0 | This plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79707 | CSK21_HUMAN_D0 | This plasmid encodes the kinase domain of CSK21. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79708 | EPHA3_HUMAN_D0 | This plasmid encodes the kinase domain of EPHA3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79709 | BMPR2_HUMAN_D0 | This plasmid encodes the kinase domain of BMPR2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79710 | M3K5_HUMAN_D0 | This plasmid encodes the kinase domain of M3K5. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79711 | KCC2G_HUMAN_D0 | This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79712 | E2AK2_HUMAN_D0 | This plasmid encodes the kinase domain of E2AK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79713 | MK01_HUMAN_D0 | This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79714 | CSKP_HUMAN_D0 | This plasmid encodes the kinase domain of CSKP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79715 | CHK2_HUMAN_D0 | This plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79716 | KC1G2_HUMAN_D0 | This plasmid encodes the kinase domain of KC1G2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79717 | DMPK_HUMAN_D0 | This plasmid encodes the kinase domain of DMPK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79718 | KCC2B_HUMAN_D0 | This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79719 | FGFR1_HUMAN_D0 | This plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79720 | KS6A1_HUMAN_D1 | This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79721 | DAPK3_HUMAN_D0 | This plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79722 | STK10_HUMAN_D0 | This plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79723 | KC1D_HUMAN_D0 | This plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79724 | KC1E_HUMAN_D0 | This plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79725 | NEK1_HUMAN_D0 | This plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79726 | CDK2_HUMAN_D0 | This plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79727 | ABL1_HUMAN_D0 | This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79728 | DAPK1_HUMAN_D0 | This plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79729 | DYRK2_HUMAN_D0 | This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79730 | HASP_HUMAN_D0 | This plasmid encodes the kinase domain of HASP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79731 | FGFR3_HUMAN_D0 | This plasmid encodes the kinase domain of FGFR3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79732 | EPHB3_HUMAN_D0 | This plasmid encodes the kinase domain of EPHB3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79733 | SLK_HUMAN_D0 | This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79734 | KCC2D_HUMAN_D0 | This plasmid encodes the kinase domain of KCC2D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79735 | NEK7_HUMAN_D0 | This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79736 | PHKG2_HUMAN_D0 | This plasmid encodes the kinase domain of PHKG2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79737 | VRK2_HUMAN_D0 | This plasmid encodes the kinase domain of VRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79738 | AAPK2_HUMAN_D0 | This plasmid encodes the kinase domain of AAPK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79739 | AURKA_HUMAN_D0 | This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79740 | MARK3_HUMAN_D0 | This plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79741 | KAPCA_HUMAN_D0 | This plasmid encodes the kinase domain of KAPCA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79742 | STK24_HUMAN_D0 | This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79743 | VGFR1_HUMAN_D0 | This plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79744 | KCC4_HUMAN_D0 | This plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79745 | KCC1G_HUMAN_D0 | This plasmid encodes the kinase domain of KCC1G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79746 | KCC2A_HUMAN_D0 | This plasmid encodes the kinase domain of KCC2A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79747 | FAK2_HUMAN_D0 | This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression. |
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| 79748 | Lambda Phosphatase | Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression. |
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| 79749 | YopH (residues 164-468) | This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression. |
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