PurposeThis plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79725||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepET His10 TEV LIC cloning vector (2B-T-10)
Backbone manufacturerScott Gradia (Addgene plasmid # 78173)
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
SpeciesH. sapiens (human)
- Promoter T7
/ Fusion Protein
- His10-TEV (N terminal on backbone)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NEK1_HUMAN_D0 was a gift from John Chodera & Nicholas Levinson & Markus Seeliger (Addgene plasmid # 79725 ; http://n2t.net/addgene:79725 ; RRID:Addgene_79725)
For your References section:An open library of human kinase domain constructs for automated bacterial expression. Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD. Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081. 10.1021/acs.biochem.7b01081 PubMed 30004690