YopH (residues 164-468)
(Plasmid #79749)

• Purpose: This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
• Depositing Labs: John Chodera, Nicholas Levinson, Markus Seeliger
• Publication: Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.

Backbone

• Vector backbone: pET co-transformation cloning vector (13S-A)
• Backbone manufacturer: Scott Gradia (Addgene plasmid # 48323)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: YopH (residues 164-468)
• Promoter: T7

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 reverse

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711