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Jannik Elverløv-Jakobsen Lab Plasmids


The Jannik Elverløv-Jakobsen Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

Learn more about research in the Jannik Elverløv-Jakobsen Lab.

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ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
67275Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIRpuromycin resistant gene (Synthetic)Mammalian Expression Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR (unpublished) For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines Amp Add to Cart
67276Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3GFP (mWasabi), hygroMammalian Expression Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR (unpublished) RMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PA Amp Add to Cart
67277LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIRfirst casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site (Other), second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoffMammalian Expression Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR (unpublished) inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system. Amp Add to Cart
67278Villinpromoter-blue-FlpOERT2mTQ2=mturquoise2 blue fluorescent gene (Other) Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR (unpublished) 4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context Amp Add to Cart
67279pdx1-mTQ2-P2A-FlpO-PAmTQ2=mturquoise2 blue fluorescent gene (Other), FlpO recombinaseMammalian Expression Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR (unpublished) Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context amp Add to Cart
68345LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIRpuromycin (Synthetic), hCas9 (Synthetic)Mammalian Expression, CRISPR ; Flp/Frt Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression Amp Add to Cart
68346AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA3xgRNA;PTENA, p53B,SMAD4A (Synthetic), FlPO (Synthetic)Mammalian Expression, AAV ; Flp/Frt Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) Used to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4 Amp Add to Cart
68347AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTRFlPO (Synthetic)Mammalian Expression, AAV Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) AAV plasmid expressing the FlpO recombinase. with empty gRNA amp Add to Cart
68348px330_PTEN exon 5 gRNAPTEN exon 5 gRNA (Synthetic)Mammalian Expression Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter. amp Add to Cart
68349px330_P53 exon 8 gRNAP53 exon 8 gRNA (Synthetic)Mammalian Expression Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter. amp Add to Cart
68350px330_SMAD exon 2 gRNASMAD exon 2 gRNA (Synthetic)Mammalian Expression Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter. amp Add to Cart
68351px330_P53 exon 7 gRNAP53 exon 7 gRNA (Synthetic)Mammalian Expression Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter. amp Add to Cart
68352px330_SMAD exon 9 gRNASMAD exon 9 gRNA (Synthetic)Mammalian Expression Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter. amp Add to Cart
683564xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 (Synthetic), FlpO recombinase (Synthetic)Mammalian Expression, CRISPR Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter Amp Add to Cart
683575xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 (Synthetic), FlpO recombinase (Synthetic)Mammalian Expression, CRISPR Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter. Amp Add to Cart
684053xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 (Synthetic), FlpO (Synthetic)Mammalian Expression, CRISPR Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter Amp Add to Cart