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pSYN-PACE-001

Plasmid

#42503

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

soluble PACE (FURIN Human)

Expression

Mammalian

Promoter

cmv

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

Plasmid

#113899

Purpose

Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

TetR-HA-NLS-P2A-BSD-Myc

Use

Lentiviral

Tags

HA-Myc

Promoter

CMV-MIE

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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Lentiviral CaMKKbeta

Plasmid

#33322

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

Flag

Promoter

human PGK

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-MCC

Plasmid

#133307

Purpose

"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

microcin-V bacteriocin cassette (cvaC E. coli)

Use

Synthetic Biology

Expression

Bacterial

Mutation

N112D (please see depositors comments)

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-syn pA

Plasmid

#99689

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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EF.CMV.RFP

Plasmid

#17619

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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p23-mNeongreen-DDX21 WT-mRuby3

Plasmid

#179225

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Tags

mNeongreen and mRuby3

Expression

Mammalian

Promoter

EF-1alpha

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[himar1C9]

Plasmid

#213906

Purpose

Mariner transposon with ATc-inducible himar1C9 transposase

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC6.DC-FLAG

Plasmid

#30483

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Catalytically inactive mutant (H216/611A)

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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TFORF1618

Plasmid

#144295

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NF1 (NF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pG20_MCS_Hyg

Plasmid

#159709

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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TBXA2R-Tango

Plasmid

#66517

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

TBXA2R (TBXA2R Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pLX317-SNAI1

Plasmid

#115446

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

SNAI1 (SNAI1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-T2TP

Plasmid

#114725

Purpose

expression in mammalian cells

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

Tol2 transposase

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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RHPMP132 (mEOS3.1-beta Actin)

Plasmid

#88836

Purpose

Localization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1

Depositor

Ricardo Henriques

Insert

beta-Actin (ACTB Human)

Tags

mEOS3.1

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMarC9-R6k

Plasmid

#89477

Purpose

transposon plasmid, compatible with Tn-Seq

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pMarC9-R6k

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP Centrin2 (Nigg UK185)

Plasmid

#41147

Depositor

Erich Nigg

Article

Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

Insert

Centrin-2 (CETN2 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBUE411-B

Plasmid

#198234

Purpose

pPLTP::BBM cassette in pBUE411

Depositor

Andrea Gallavotti

Article

Chen et al bioRxiv 2022.09.02.506370

Insert

pPLTP::BBM cassette

Use

CRISPR; Plant binary vector

Expression

Plant

Promoter

PLTP

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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GUM BUB3

Plasmid

#84029

Purpose

Expression of mCherry tagged BUB3 in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BUB3 (BUB3 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

UCOE / SFFV

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP

Plasmid

#32149

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

FRT> STOP STOP FRT>

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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