169,546 results

1. CoV2-N-R203M

   Plasmid

   #177952

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (R203M) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R203M

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pdCas9-DNMT3A-PuroR

   Plasmid

   #71667

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1.D2-NP

   Plasmid

   #134366

   Purpose

   Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   D2-NP (DRD2 Human)
   

   Use

   Tags

   Flag, natural peptide of nanoluciferase, and sign…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-OTp-GCaMP6s

   Plasmid

   #192945

   Purpose

   To drive GCaMP6s under the control of mouse oxytocin promoter

   Depositor

   Kazunari Miyamichi

   Article

   Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSFDuet-1_VNp-mNeongreen-StefinA

   Plasmid

   #182419

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-stefin (CSTA Human)
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET N6his GFP1-9

   Plasmid

   #182240

   Purpose

   Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

   Depositor

   Stéphanie Cabantous

   Article

   Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

   Insert

   GFP1-9
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 7TFC

   Plasmid

   #24307

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-FFluc // SV40-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

   Plasmid

   #231986

   Purpose

   Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   OVA-2A-ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PL-SIN-Oct4-EGFP

   Plasmid

   #21319

   Purpose

   Lentiviral vector, expresses EGFP under Oct3/4 promoter

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Oct-4 promoter region

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-cytoFLARE1.0-TEV

    Plasmid

    #234517

    Purpose

    Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    EGFP-P2A-CaM-V5-TEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pLX208-cytoFLARE1.0-TF

    Plasmid

    #234513

    Purpose

    Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    ERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pT7C-HSP90AB1

    Plasmid

    #177659

    Purpose

    Recombinant protein synthesis of human HSP90AB1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSP90AB1 (HSP90AB1 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p5-71

    Plasmid

    #118085

    Purpose

    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid

    Depositor

    Bryan Dickinson

    Article

    Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

    Insert

    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1/Ndi-1/myc-His

    Plasmid

    #127503

    Purpose

    Expresses myc-tagged yeast Ndi-1 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    Ndi-1 (NDI1 Budding Yeast)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFRT/FLAG/HA-DEST PUM2

    Plasmid

    #40292

    Depositor

    Thomas Tuschl

    Article

    Hafner et al Cell. 2010 Apr 2;141(1):129-41.

    Insert

    Pumilio homolog 2 (PUM2 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAVS1-TRE-SHH

    Plasmid

    #134350

    Purpose

    Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

    Depositor

    Lorenz Studer

    Article

    Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

    Insert

    SHH (SHH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pISH-Pax6

    Plasmid

    #74350

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pax6. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    paired box 6 (Pax6 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-mCherry-WPRE

    Plasmid

    #127860

    Purpose

    pAAV plasmid expressing mCherry under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)-OTI-TCRB_p2_TCRA

    Plasmid

    #131034

    Purpose

    Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRB (Tcrb Mouse)
    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-OptoCAR-mScarlet

    Plasmid

    #170463

    Purpose

    Expresses an optically-controllable chimeric antigen receptor that is dependent on dimerizer drug, fused to mScarlet fluorophore

    Depositor

    John James

    Article

    Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

    Insert

    OptoCAR
    

    Use

    Lentiviral

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge