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Plasmid#167500PurposeExpression of dual inducible Cas9 with CMV promoterDepositorInsertCas9
UseCRISPR, Lentiviral, and LuciferaseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLCKO2
Plasmid#125518PurposeLentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET264-pUC 24xMS2V6 Loxp KANr Loxp
Plasmid#104393PurposeYeast endogenous mRNA taggingDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET N6his GFP1-9
Plasmid#182240PurposeTripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.DepositorInsertGFP1-9
UseTags6HISExpressionBacterialMutationPromoterT7Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP Turquoise2-Syntaxin17
Plasmid#89938PurposeExpresses Syntaxin17 tagged with Turquoise2 in mammalian cellsDepositorInserthuman Syntaxin 17 (STX17 Human)
UseRetroviralTagsmTurquoise2ExpressionMutationPromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
TadCBE-Cas9NG
Plasmid#225093PurposePlasmid for bacterial purification of codon-optimized TadCBE-Cas9NGDepositorInsertTadCBE-Cas9NG
UseCRISPRTags8xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGTMV
Plasmid#118755PurposeBinary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.DepositorInsertTobacco mosaic virus
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SPI1_P2A_Hygro_Barcode
Plasmid#120482PurposeBarcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSPI1 (SPI1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Membrane mNeonGreen
Plasmid#198057PurposePlasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane mNeonGreen (LCK A.victoria, Human)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmNeonGreenExpressionMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOpen-GBDpol
Plasmid#165501PurposeRobust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.DepositorInsertPyrococcus Sp. Heat-Stable DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKH3
Plasmid#12555DepositorTypeEmpty backboneUseTags3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-BCL2
Plasmid#116717PurposeLentiviral expression of BCL2DepositorInsertBCL2 (BCL2 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDESTtol2pACrymCherry
Plasmid#64023PurposeGateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lensDepositorInsertmCherry
UseGatewayTagsExpressionMutationPromoteralpha-Acrystallin promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-HAPLN1
Plasmid#200777PurposeExpresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoterDepositorInsertHyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
UseAAVTagscfGFPExpressionMutationPromoterSynapsinAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-Ecad
Plasmid#205188PurposeThis vector encodes of a synCAM (Ecad) and GFPDepositorInsertsynCAM Ecad and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-TBG-Cyto-TurboID
Plasmid#149414PurposeHepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertCyto-TurboID
UseAAVTagsV5ExpressionMutationPromoterTBGAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBR322-PVCpnf18-21
Plasmid#198273PurposeContains only regulatory genes; for generating empty PVCsDepositorInsertPVCpnf18-21
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
G-CaMP3
Plasmid#22692Purposea single-wavelength GCaMP2-based genetically encoded calcium indicatorDepositorInsertG-CaMP3
UseTagsExpressionMammalianMutationN363D mutation (increased fluorescence)PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only