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182,008 results

Showing: 11621 - 11640 of 182008 results

  7. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Publication
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80424-plasmid-map-sequence-id-151441

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  9. pcDNA5-FRT-TO-EGFP-AID

    Plasmid
    #80075
    Purpose
    Mammalian expression vector for N-terminal EGFP-AID tagging
    Depositor
    Andrew Holland
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-AID
    Tags
    AID and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80075-plasmid-map-sequence-id-150337

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  14. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  15. pTC217

    Plasmid
    #70018
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70018-plasmid-map-sequence-id-124557

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  18. Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2

    Plasmid
    #69623
    Purpose
    A FRET sensor for Galphai1 activation, encoded on a single plasmid.
    Depositor
    Dorus Gadella
    Publication
    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Gbeta-2A-cpV-Ggamma2
    Galphai-mTurquoise2
    Tags
    ciruclar permutated Venus (cp173V) and mTurquoise…
    Expression
    Mammalian
    Promoter
    CMV and CMV (IRES)
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69623-plasmid-map-sequence-id-459280

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  20. pCDH-EF1-DIO-tdTomato

    Plasmid
    #72254
    Purpose
    Express tdTomato under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    72254-plasmid-map-sequence-id-134462

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Showing: 11621 - 11640 of 182008 results