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pTRE-GFP-NMHC II-A
(Plasmid #10844)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10844 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NMHC II-A
  • Alt name
    nonmuscle myosin II-A heavy chain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6000
  • Entrez Gene
    MYH9 (a.k.a. BDPLT6, DFNA17, EPSTS, FTNS, MATINS, MHA, NMHC-II-A, NMMHC-IIA, NMMHCA)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The CMV promoter in pEGFP-C3 is replaced by a tet-responsive promoter amplified from pTRE (Clontech). NMHC II-A can be released by HindIII/SpeI digestion.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE-GFP-NMHC II-A was a gift from Robert Adelstein (Addgene plasmid # 10844 ; http://n2t.net/addgene:10844 ; RRID:Addgene_10844)
  • For your References section:

    Conditional expression of a truncated fragment of nonmuscle myosin II-A alters cell shape but not cytokinesis in HeLa cells. Wei Q, Adelstein RS. Mol Biol Cell. 2000 Oct . 11(10):3617-27. 10.1091/mbc.11.10.3617 PubMed 11029059