|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15983||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 4300
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
Copy numberHigh Copy
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Template vector for a PCR based gene-tagging (5 x FLAG tag) method in S. pombe.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFA6a-5FLAG-KanMX6 was a gift from Eishi Noguchi (Addgene plasmid # 15983 ; http://n2t.net/addgene:15983 ; RRID:Addgene_15983)
For your References section:A vector system for genomic FLAG epitope-tagging in Schizosaccharomyces pombe. Noguchi C, Garabedian MV, Malik M, Noguchi E. Biotechnol J. 2008 Aug 26. ():. 10.1002/biot.200800140 PubMed 18729046