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pFA6a-5FLAG-natMX6
(Plasmid #19343)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19343 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a-5FLAG-kanMX6
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Yeast Expression
  • Selectable markers
    nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    natMX6
  • Species
    Streptomyces noursei
  • Insert Size (bp)
    1200

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site PmeI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are a few point mutations in drug resistant genes. These mutations are derived from the original drug resistant plasmids we have obtained. However these mutations do not alter drug resistant property of the genes or the functions of our plasmids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-5FLAG-natMX6 was a gift from Eishi Noguchi (Addgene plasmid # 19343 ; http://n2t.net/addgene:19343 ; RRID:Addgene_19343)
  • For your References section:

    A vector system for genomic FLAG epitope-tagging in Schizosaccharomyces pombe. Noguchi C, Garabedian MV, Malik M, Noguchi E. Biotechnol J. 2008 Aug 26. ():. 10.1002/biot.200800140 PubMed 18729046