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A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing.
Tong Y, Jorgensen TS, Whitford CM, Weber T, Lee SY
Nat Commun. 2021 Sep 1;12(1):5206. doi: 10.1038/s41467-021-25541-3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
172715pCRISPR-PE-bacteriaExpression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing
172716pPEgRNADelivery of guide RNA for prime editing
172717pnsgRNADelivery of guide RNA for prime editing
172718pCDF-GFPplusExpression of a fast folding GFP variant GFP plus

Antibodies from Article