Identification and characterization of essential genes in the human genome.

Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES, Sabatini DM
Science. 2015 Oct 15. pii: aac7041. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
70265	pMXs-C3orf17-gfp	Expresses C3orf17 in mammalian cells with GFP reporter.
70266	pMXs-C9orf114-gfp	Expresses C9orf114 in mammalian cells with GFP reporter.
70645	pMXs-C16orf80-flag	Expresses C16orf80 in mammalian cells with FLAG-tag epitope.
70646	pMXs-C3orf17-flag	Expresses C3orf17 in mammalian cells with FLAG-tag epitope.
70647	pMXs-C9orf114-flag	Expresses C9orf114 with FLAG-tag epitope.
70648	pMXs-DDX3X	Expresses WT DDX3X in mammalian cells under blasticidin selection.
70649	pTagRFP-C1-Fibrillarin	Expresses Fibrillarin nucleolar marker with TurboRFP reporter.
70650	lentiCRISPR - C16orf80 sgRNA 2	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
70651	lentiCRISPR - C16orf80 sgRNA 1	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
70652	lentiCRISPR - C3orf17 sgRNA 1	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
70653	lentiCRISPR - C3orf17 sgRNA 2	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
70654	lentiCRISPR - C9orf114 sgRNA 2	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
70655	lentiCRISPR - C9orf114 sgRNA 1	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
70656	lentiCRISPR - DDX3Y sgRNA 1	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
70657	lentiCRISPR - DDX3Y sgRNA 2	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
70658	lentiCRISPR - Amplicon, BCR/ABL sgRNA 2	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.
70659	lentiCRISPR - Amplicon, BCR/ABL sgRNA 1	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone
70660	lentiCRISPR - Amplicon, JAK2 sgRNA 2	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
70661	lentiCRISPR - AAVS1 sgRNA	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone
70662	lentiCRISPR - CTRL sgRNA	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone
70679	lentiCRISPR - Amplicon, JAK2 sgRNA 1	Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone