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biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes.
Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 10.1073/pnas.1604935113. Epub 2016 Apr 25.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
80610pLIBpLIB - Library vector (biGBac system for expression of protein complexes in insect cells)
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80611pBIG1apBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)
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80612pBIG1bpBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)
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80613pBIG1cpBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)
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80614pBIG1dpBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)
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80615pBIG1epBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)
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80616pBIG2abpBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)
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80617pBIG2abcpBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)
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80618pBIG2abcdpBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)
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80619pBIG2abcdepBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)
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