Purpose(Empty Backbone) pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80617||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5669
Vector typeInsect Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
- Cloning method Gibson Cloning
- 5′ sequencing primer TCAACAGGTTGAACTGCTGATC
- 3′ sequencing primer GGTGTAGCGTCGTAAGCTAATAC (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBIG2abc was a gift from Jan-Michael Peters (Addgene plasmid # 80617 ; http://n2t.net/addgene:80617 ; RRID:Addgene_80617)
For your References section:biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes. Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM. Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 10.1073/pnas.1604935113. Epub 2016 Apr 25. 10.1073/pnas.1604935113 PubMed 27114506