PurposeVector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80811||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3956
- Total vector size (bp) 6450
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
Copy numberHigh Copy
Insert Size (bp)2494
/ Fusion Protein
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pScarlessHD-sfGFP-DsRed was a gift from Kate O'Connor-Giles (Addgene plasmid # 80811 ; http://n2t.net/addgene:80811 ; RRID:Addgene_80811)