PurposeTransient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68416||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
- Promoter human EF1[alpha]
/ Fusion Protein
- 3xFLAG (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer EF-1a-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Contains NLS sequences at the N- and C-terminus of the dCas9 protein. The C-terminal NLS immediately preceedes the 3xFLAG tag
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEF_dCas9 was a gift from John Rinn (Addgene plasmid # 68416 ; http://n2t.net/addgene:68416 ; RRID:Addgene_68416)
For your References section:Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. 10.1038/nmeth.3433 PubMed 26030444