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John Rinn Lab Plasmids


The John Rinn Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
68414pLenti_9xD3B_Cluc_Norm Cypridina Luciferase-2A-Venus YFP (Other)Lentiviral, LuciferaseMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format. pLenti6.3/TO/V5-DEST Add to Cart
68415pTrans_9xD3B_Cluc_Norm Cypridina Luciferase-2A-Venus YFP (Other)Mammalian Expression, LuciferaseMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format. pNEB193 Add to Cart
68416pEF_dCas9dCas9 (Other)Mammalian Expression, CRISPRMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter. pNEB193
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)
  • Add to Cart
    68417pEF_dCas9-VP64dCas9 (Other)Mammalian Expression, CRISPRMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter. pNEB193
  • Tags / Fusion Proteins
    • 3xFLAG (C terminal on insert)
    • VP64 (C terminal on insert)
  • Add to Cart
    68418pUBC_HA_2xPP7-VP64PP7(deltaFG) (Other)Mammalian Expression, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter pNEB193
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • VP64 (C terminal on insert)
  • Add to Cart
    68419pCMV_mCherry_2A_HA_2xMS2-VP64MS2 (Other)Mammalian Expression, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter pcDNA3.1(+)
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • VP64 (C terminal on insert)
  • Add to Cart
    68420pUBC_V5_MS2-mCherryMS2 (Other)Mammalian Expression, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter pNEB193
  • Tags / Fusion Proteins
    • V5 (N terminal on insert)
    • mCherry (C terminal on insert)
  • Add to Cart
    68421pUBC_HA_L7Ae-VP64L7Ae (Other)Mammalian Expression, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter pNEB193
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • VP64 (C terminal on insert)
  • Add to Cart
    68422pU6_(GLuc)_sgRNAGluc sgRNA (Synthetic)Mammalian Expression, CRISPRMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter pNEB193 Add to Cart
    68423pU6_(GLuc)_TOP1TOP1 construct (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter pNEB193 Add to Cart
    68424pU6_(GLuc)_INT[3xPP7_SL]INT construct bearing three PP7 Stem-loops (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter pNEB193 Add to Cart
    68425pU6_(GLuc)_INT[S1Apt]INT construct with S1 streptavidin aptamer (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter pNEB193 Add to Cart
    68426pU6_(GLuc)_INT[3xMS2_SL] INT construct_bearing three MS2 Stem-loops (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter pNEB193 Add to Cart
    68428pU6_(GLuc)_INT[GFPApt] INT construct_bearing the GFP aptamer (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoter pNEB193 Add to Cart
    68429pU6_(GLuc)_INT[3xK-T] INT construct_bearing three kink-turns (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter pNEB193 Add to Cart
    68430pU6_(GLuc)_INT[Spinach2] INT construct_bearing the "Spinach2" aptamer (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter pNEB193 Add to Cart
    68431pU6_(GLuc)_INT[BoBS] INT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoter pNEB193 Add to Cart
    68432pU6_(GLuc)_INT[P4-P6(3xPP7)] INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops) (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter pNEB193 Add to Cart
    68433pU6_(Gluc)_INT(GenPurpClon)INT construct (Synthetic)Mammalian Expression, CRISPR ; Cloning vector for generating INT-like constructsMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. General Purpose cloning vector for "INT"-like constructs under U6-promoter expression. pNEB193 Add to Cart
    68434pCMV/3' Box_(GLuc)_TOP1TOP1 construct (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone. pNEB193 Add to Cart
    68435pCMV/3' Box_(GLuc)_TOP2TOP2 construct (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone. pNEB193 Add to Cart
    68436pCMV/3' Box_(GLuc)_INT INT construct_bearing three PP7 Stem-loops (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone. pNEB193 Add to Cart
    68437pU1/Sm/3' Box_(GLuc)_TOP1TOP1 construct (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone. pNEB193 Add to Cart
    68438pU1/Sm/3' Box_(GLuc)_INT INT construct_bearing three PP7 Stem-loops (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone. pNEB193 Add to Cart
    68439pCMV/MASC_(GLuc)_TOP1TOP1 construct (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone. pNEB193 Add to Cart
    68440pCMV/MASC_(GLuc)_INT INT construct_bearing three PP7 Stem-loops (Synthetic)Mammalian Expression, CRISPR, Synthetic BiologyMultiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone. pNEB193 Add to Cart