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Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I
ACS Synth Biol. 2016 Dec 19.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
84608pCRISPRyl_AXPIntroduces DSB at AXP locus in Y. lipolytica
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84609pCRISPRyl_XPR2Introduces DSB at XPR2 locus in Y. lipolytica
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84610pCRISPRyl_A08Introduces DSB at A08 locus in Y. lipolytica
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84611pCRISPRyl_D17Introduces DSB at D17 locus in Y. lipolytica
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84612pCRISPRyl_MFE1Introduces DSB at MFE1 locus in Y. lipolytica
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84613pHR_AXP_hrGFPHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus
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84614pHR_XPR2_hrGFPHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus
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84615pHR_A08_hrGFPHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus
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84616pHR_D17_hrGFPHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus
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84617pHR_MFE1_hrGFPHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus
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