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Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human.
Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
60432pPB-hCMV1-hNANOG-IVtet-inducible human NANOG
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60433pPB-hCMV1-hKLF2-IVtet-inducible human KLF4
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60434pPB-PGK-hESRRBOver-expression of human ESRRB
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60435pPB-PGK-hKLF4Over-expression of human KLF4
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60436pPB-PGK-destinationDestination vector with PGK - Hygromycin selection marker
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60439pPB-EOS-GFP-IPPiggyBac for EOS-GFP
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60440PpyCAG-NANOG-IBTransient human NANOG expression
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60441PpyCAG-KLF2-IBTransient human KLF2 expression
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60612pPB-CAG-rtTA-INBackbone for tet-inducible genes
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