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plentiCRISPRv2 DLG5

Plasmid

#258468

Purpose

CRISPR-mediated knock-out of human DLG5

Depositor

Matthew Kutys

Article

Mayo et al J Cell Biol. 2026 Jun 1;225(6):e20250314

Insert

DLG5 sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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Redi.3.SaCas9-V16A-I934K-L1035V

Plasmid

#235232

Purpose

Expresses reduced immunogenicity variant of SaCas9 (Redi.3.SaCas9) with mutations V16A, I934TK L1035V. Has AAV ITRs

Depositor

Feng Zhang

Article

Raghavan et al Nat Commun. 2025 Jan 2;16(1):105. doi: 1

Insert

hSaCas9

Use

AAV and CRISPR

Expression

Mammalian

Mutation

V16A, I934K, L1035V

Available Since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pCC602 - U6-sgAAVS1-EF1a-Cas9-2a-Puro

Plasmid

#258563

Purpose

U6-driven AAVS1 guide RNA and EF-1α-driven puromycin resistance cassette for PAIR all-in-one AAVS1 knock-in

Depositor

Siyuan Zhang

Article

Chang et al bioRxiv [Preprint]. 2026 May 8:2026.05.0

Insert

AAVS1sgRNA spacer

Use

CRISPR

Expression

Mammalian

Available Since

July 14, 2026

Availability

Academic Institutions and Nonprofits only

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pVC0*_A_14_Acarb J23106 (mScarlet-I)

Plasmid

#250029

Purpose

ABR, lv0*, Acarb, mScarlet-I

Depositor

Georg Fritz

Article

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

ABR, lv0*, Acarb, mScarlet-I

Use

Synthetic Biology

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pVC0*_A_12_Acarb J23106 (sfGFP)

Plasmid

#250028

Purpose

ABR, lv0*, Acarb, sfGFP

Depositor

Georg Fritz

Article

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

ABR, lv0*, Acarb, sfGFP

Use

Synthetic Biology

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pPD770 Tet-On 3G in TUPV3

Plasmid

#253883

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and TetOn3G

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

TetOn3G

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD847 Tet On 3G in TU2

Plasmid

#253884

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and TetOn3G

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

TetOn3G

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1043 TRE3GV mTagBFP2

Plasmid

#253903

Purpose

Components for Integration Vectors: mMoClo TUPV, with TRE3GV promoter and mTagBFP2 in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GV

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pANC76(TVMVp-IRES-dTomato)

Plasmid

#248362

Purpose

Lentiviral vector that can express TVMVp. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

TVMVp

Use

Lentiviral

Promoter

tight TRE

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-dCas9(sa)-VP64-AAV

Plasmid

#213035

Purpose

dCas9-VP64 expressed under control of TRE

Depositor

Nadav Ahituv

Article

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

dCas9

Use

AAV and CRISPR

Tags

NLS and NLS-VP64

Promoter

TRE, miniCMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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EF1as-ZIM3-Cas9-P2A-GFP

Plasmid

#239603

Purpose

Constitutive active CRISPRgenee construct

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA032

Plasmid

#240781

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA031

Plasmid

#240780

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA030

Plasmid

#240779

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_low - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA023

Plasmid

#240772

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA022

Plasmid

#240771

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA021

Plasmid

#240770

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_low - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA015

Plasmid

#240764

Purpose

Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA p15A PrhaBAD BCD_low - gfp - tetAp15A- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA014

Plasmid

#240763

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 PrhaBAD BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA013

Plasmid

#240762

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 PrhaBAD BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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