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pET28 mePOU-POU

Plasmid

#206392

Purpose

Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Tags

6xHis

Expression

Bacterial

Mutation

POU DNA binding domain only

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL28_Cas9-His Δcys E532C/E1207C

Plasmid

#206289

Purpose

Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S, E1207C

Promoter

T7

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDL27_Cas9-His Δcys E532C/E945C

Plasmid

#206288

Purpose

Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S, E945C

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL26_Cas9-His Δcys M1C/E532C

Plasmid

#206287

Purpose

Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

M1C, C80S, E532C, C574S

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H10L

Plasmid

#198683

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI1_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H10R

Plasmid

#198684

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI1_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H09R

Plasmid

#198681

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII4_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H09L

Plasmid

#198682

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII4_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H06R

Plasmid

#198676

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII5_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H03L

Plasmid

#198669

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII2_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H01R

Plasmid

#198666

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

X3_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H11R

Plasmid

#198686

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII1_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H11L

Plasmid

#198685

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII1_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H08R

Plasmid

#198680

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI5_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H08L

Plasmid

#198679

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI5_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H07R

Plasmid

#198678

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

X2_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H07L

Plasmid

#198677

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

X2_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H06L

Plasmid

#198675

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII5_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H05R

Plasmid

#198674

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI3_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.H05L

Plasmid

#198673

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI3_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge