We narrowed to 2,140 results for: dcas9

1. pMT423

   Plasmid

   #139382

   Purpose

   Constitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Constitutively expressed dCas9, LacI and TetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   P1

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVC-Ds-MCS-Ds

   Plasmid

   #102416

   Purpose

   Empty vector with a multiple cloning site flanked by Ds integration elements

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEVA221pmCDA1-T7RNAP

   Plasmid

   #167978

   Purpose

   Expresses fusion protein pmCDA1-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   pmCDA1-T7RNAP (T7p07 Synthetic)
   

   Use

   Tags

   T7 tag and T7RNAP

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-Ptet

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

   Plasmid

   #84248

   Purpose

   Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgTRE3G
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

   Plasmid

   #84250

   Purpose

   Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sa sgTRE3G
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGH153_MS2-AIDΔ-Hygro

   Plasmid

   #85416

   Purpose

   lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   MS2-AIDΔ and Hygromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCas2C

   Plasmid

   #82374

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEVA221rAPOBEC1-T7RNAP

   Plasmid

   #167979

   Purpose

   Expresses fusion protein rAPOBEC1-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   rAPOBEC1-T7RNAP (T7p07 Synthetic)
   

   Use

   Tags

   T7 tag and T7RNAP

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-Ptet

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NAX_CAGGS_CAS9_m4_VPR_KanR neo

   Plasmid

   #167885

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFD115

    Plasmid

    #124768

    Purpose

    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. NAX_CAGGS_CAS9_m4_VPR_mcherry

    Plasmid

    #167886

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

12. pGH183_MS2-AIDΔDead-Hygro

    Plasmid

    #85414

    Purpose

    lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AIDΔDead and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NAX_CAGGS_CAS9_m4_Blasto

    Plasmid

    #167864

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLH-sgRNA1-boxB-MS2-PP7

    Plasmid

    #75397

    Purpose

    sgRNA1-boxB-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-boxB-MS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-gRNA-puro

    Plasmid

    #180426

    Purpose

    Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    gRNAscr
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLH-sgRNA1-MS2-PP7

    Plasmid

    #75392

    Purpose

    sgRNA1-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XMS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEVA221MBPc-AID-T7RNAP

    Plasmid

    #167975

    Purpose

    Expresses fusion protein MBPc-AID-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    MBPc-AID-T7RNAP
    

    Use

    Tags

    MBPc and T7RNAP

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-Ptet

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGH156_MS2-AID-Hygro

    Plasmid

    #85415

    Purpose

    lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AID and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NAX_CAGGS_CAS9_m4_KRAB_KanR neo

    Plasmid

    #167877

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NAX_CAGGS_CAS9_m4_Puro

    Plasmid

    #167871

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge