We narrowed to 13,003 results for: CAR

1. pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A

   Plasmid

   #169826

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   T456A S1406A S1859A; TCCC -> AGTC silent mutat…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-CMV-PE2-IRES-ZeoR

   Plasmid

   #207352

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   Prime editor 2 (PE2) enzyme
   

   Use

   Lentiviral

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

   Plasmid

   #160628

   Purpose

   Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
   

   Use

   CRISPR

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos, 35S, 35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINO_NbH11-H4

   Plasmid

   #155364

   Purpose

   Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

   Depositor

   Ray Owens

   Article

   Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

   Insert

   H11-H4
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pOPINO_NbH11-D4

   Plasmid

   #155363

   Purpose

   Expresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

   Depositor

   Ray Owens

   Article

   Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

   Insert

   Nb H11-D4
   

   Tags

   His6

   Promoter

   T7

   Available Since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

   Plasmid

   #112060

   Purpose

   Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

   Depositor

   Amy Palmer

   Article

   Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

   Insert

   NES-ZapCV2
   

   Tags

   Nuclear Export Signal (NES) derived from HIV-1

   Expression

   Mammalian

   Mutation

   ECFP gene is truncated 36 bp at 3' end. Venu…

   Promoter

   CMV

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

   Plasmid

   #207355

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
   

   Use

   Lentiviral

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-BLAST EGFP-flag-CAD

   Plasmid

   #188118

   Purpose

   Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   EGFP tag and Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. tetO-HEY2

   Plasmid

   #170691

   Purpose

   doxycycline-inducible overexpression of HEY2

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   HEY2 – hes related family bHLH transcription factor with YRPW motif 2 (HEY2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

    Plasmid

    #160567

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINO_H3

    Plasmid

    #171927

    Purpose

    Bacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_H3
    

    Tags

    His6

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pPB CAG SMARCA4 T910M-Venus

    Plasmid

    #153951

    Purpose

    piggyBac transposon vector with CAG promoter expressing SMARCA4 (T910M mutant)-Venus fusion protein

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    T910M

    Promoter

    CAG

    Available Since

    Aug. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINO_C5

    Plasmid

    #171925

    Purpose

    Bacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_C5
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pMXS-IRES-BLAST Halo-flag-CAD

    Plasmid

    #188117

    Purpose

    Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag and Halo tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOPINO_C1

    Plasmid

    #171924

    Purpose

    Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_C1
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pOPINO_F2

    Plasmid

    #171926

    Purpose

    Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_F2
    

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pMXS-IRES-BLAST flag-CAD hCPS1 AD

    Plasmid

    #188130

    Purpose

    Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXS-IRES-BLAST flag-CAD E1368A

    Plasmid

    #188141

    Purpose

    Expresses C-terminal flag-tagged human CAD E1368A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXS-IRES-BLAST flag-CAD S1396A

    Plasmid

    #188126

    Purpose

    Expresses C-terminal flag-tagged human CAD S1396A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB CAG SMARCA4 T910M-IRES-EGFP

    Plasmid

    #153949

    Purpose

    piggyBac transposon vector with CAG promoter expressing T910M mutant SMARCA4 (FLAG-tagged) and EGFP

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T910M

    Promoter

    CAG

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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