A molecular switch for coordinating kinesin and dynein transport of mitochondrial cargo.
Gladkova C, Paez-Segala MG, Grant WP, Kittisopikul M, Myers SA, Wang Y, Vale RD
Science. 2026 Aug 6;393(6811):601-606. doi: 10.1126/science.aeh1475. Epub 2026 Aug 6.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248233 | pHR Miro1 (1-590) m-sfGFP FLAG I3-01 | Encodes the cytosolic portion of human Miro-1 (RHOT1) C-terminally fused with monomerized sfGFP (V206R), FLAG and the self-assembling I3-01 domain. |
| 248234 | pHR TRAK1 (1-702) mScarlet | Encodes TRAK1 (1-702) C-terminally fused with mScarlet. Expression of this fragment facilitates TRAK1-mediated motility of Miro-1 decorated nanocages in cells. |
| 248235 | pHR TRAK2 (1-700) mScarlet | Encodes TRAK2 (1-700) C-terminally fused with mScarlet. Expression of this fragment facilitates TRAK2-mediated motility of Miro-1 decorated nanocages in cells. |
| 248236 | pHR Miro1 (1-590) Halo FLAG I3-01 | Encodes the cytosolic portion of human Miro-1 (RHOT1) C-terminally fused with monomerized Halo, FLAG and the self-assembling I3-01 domain. |
| 248237 | pHR TRAK1 E242R (1-702) mScarlet | Encodes TRAK1 E242R (1-702) C-terminally fused with mScarlet. The E242R mutation disrupts kinesin-mediated transport. |
| 248238 | pHR TRAK2 E242R (1-700) mScarlet | Encodes TRAK2 E242R (1-700) C-terminally fused with mScarlet. The E242R mutation disrupts kinesin-mediated transport. |
| 248239 | pHR TRAK1 L92A, F95A (1-702) mScarlet | Encodes TRAK1 L92A, F95A (1-702) C-terminally fused with mScarlet. The L92A, F95A mutation disrupts the dynein regulatory helix (DRH). |
| 248240 | pHR TRAK2 F92A, M95A (1-700) mScarlet | Encodes TRAK2 F92A, M95A (1-700) C-terminally fused with mScarlet. The F92A, M95A mutation disrupts the dynein regulatory helix (DRH). |
| 248241 | pHR TRAK2 A129V, A130V (1-700) mScarlet | Encodes TRAK2 A129V, A130V (1-700) C-terminally fused with mScarlet. The CC1-box A129V, A130V mutations disrupts DLIC association. |
| 248242 | pHR TRAK2 d373-380 (1-700) mScarlet | Encodes TRAK2 d373-380 (1-700) C-terminally fused with mScarlet. The Spindly motif 373-380 deletion disrupts dynactin cofactor association. |
| 248243 | pHR TRAK1 A129V, A130V (1-702) mScarlet | Encodes TRAK1 A129V, A130V (1-702) C-terminally fused with mScarlet. The CC1-box A129V, A130V mutations disrupts DLIC association. |
| 248244 | pHR TRAK1 d373-380 (1-702) mScarlet | Encodes TRAK1 d373-380 (1-702) C-terminally fused with mScarlet. The Spindly motif 373-380 deletion disrupts dynactin cofactor association. |
| 248245 | pHR TRAK2 S84A (1-700) mScarlet | Encodes TRAK2 S84A (1-700) C-terminally fused with mScarlet. The S84A mutation prevents S84 phosphorylation. |
| 248246 | pHR TRAK2 S84E (1-700) mScarlet | Encodes TRAK2 S84E (1-700) C-terminally fused with mScarlet. The S84E mutation mimics S84 phosphorylation. |
| 248247 | pHR TRAK1 (1-702) mScarlet, resi 81-106 from TRAK2 | Encodes TRAK1 (1-702) C-terminally fused with mScarlet. Residues 81-106 corresponding to the dynein regulatory helix are from TRAK2. |
| 248248 | pHR TRAK2 (1-700) mScarlet, resi 81-106 from TRAK1 | Encodes TRAK2 (1-700) C-terminally fused with mScarlet. Residues 81-106 corresponding to the dynein regulatory helix are from TRAK1. |
| 248249 | pHR TRAK1 L92A, F95A, d373-380 (1-702) mScarlet | Encodes TRAK1 L92A, F95A, d373-380 (1-702) C-terminally fused with mScarlet. |
| 248250 | pHR TRAK2 F92A, M95A, d373-380 (1-700) mScarlet | Encodes TRAK2 F92A, M95A, d373-380 (1-700) C-terminally fused with mScarlet. |
| 248251 | pHR TRAK1 T84E (1-702) mScarlet | Encodes TRAK1 T84E (1-702) C-terminally fused with mScarlet. The T84E mutation mimics S84 phosphorylation. |
| 248252 | pHR TRAK1 T84A (1-702) mScarlet | Encodes TRAK1 T84A (1-702) C-terminally fused with mScarlet. The T84A mutation prevents T84 phosphorylation. |
| 248253 | pHR TRAK1 (1-702) mScarlet FKBP | Encodes TRAK1 (1-702) C-terminally fused with mScarlet and FKBP. Expression of this fragment facilitates TRAK1-mediated motility of FRB decorated nanocages in cells in the presence of rapamycin. |
| 248254 | pHR TRAK2 (1-700) mScarlet FKBP | Encodes TRAK2 (1-700) C-terminally fused with mScarlet and FKBP. Expression of this fragment facilitates TRAK2-mediated motility of FRB decorated nanocages in cells in the presence of rapamycin. |
| 248255 | pHR TRAK1 L92A, F95A (1-702) mScarlet FKBP | Encodes TRAK1 L92A, F95A (1-702) C-terminally fused with mScarlet and FKBP. The L92A, F95A mutation disrupts the dynein regulatory helix (DRH). |
| 248256 | pHR TRAK1 A129V, A130V (1-702) mScarlet FKBP | Encodes TRAK1 A129V, A130V (1-702) C-terminally fused with mScarlet and FKBP. The CC1-box A129V, A130V mutations disrupts DLIC association. |
| 248257 | pHR TRAK1 d373-380 (1-702) mScarlet FKBP | Encodes TRAK1 d373-380 (1-702) C-terminally fused with mScarlet and FKBP. The Spindly motif 373-380 deletion disrupts dynactin cofactor association. |
| 248258 | pHR TRAK1 L92A, F95A, d373-380 (1-702) mScarlet FKBP | Encodes TRAK1 L92A, F95A, d373-380 (1-702) C-terminally fused with mScarlet and FKBP. |
| 248259 | pHR TRAK1 (1-702) mScarlet FKBP, resi 81-106 from TRAK2 | Encodes TRAK1 (1-702) C-terminally fused with mScarlet and FKBP. Residues 81-106 corresponding to the dynein regulatory helix are from TRAK2. |
| 248260 | pHR TRAK2 F92A, M95A (1-700) mScarlet FKBP | Encodes TRAK2 F92A, M95A (1-700) C-terminally fused with mScarlet and FKBP. The F92A, M95A mutation disrupts the dynein regulatory helix (DRH). |
| 248261 | pHR TRAK2 A129V, A130V (1-700) mScarlet FKBP | Encodes TRAK2 A129V, A130V (1-700) C-terminally fused with mScarlet and FKBP. The CC1-box A129V, A130V mutations disrupts DLIC association. |
| 248262 | pHR TRAK2 d373-380 (1-700) mScarlet FKBP | Encodes TRAK2 d373-380 (1-700) C-terminally fused with mScarlet and FKBP. The Spindly motif 373-380 deletion disrupts dynactin cofactor association. |
| 248263 | pHR TRAK2 S84A (1-700) mScarlet FKBP | Encodes TRAK2 S84A (1-700) C-terminally fused with mScarlet and FKBP. The S84A mutation prevents S84 phosphorylation. |
| 248264 | pHR TRAK2 S84E (1-700) mScarlet FKBP | Encodes TRAK2 S84E (1-700) C-terminally fused with mScarlet and FKBP. The S84E mutation mimics S84 phosphorylation. |
| 248265 | pHR TRAK2 F92A, M95A, d373-380 (1-700) mScarlet FKBP | Encodes TRAK2 F92A, M95A, d373-380 (1-700) C-terminally fused with mScarlet and FKBP. |
| 248266 | pHR TRAK2 (1-700) mScarlet FKBP, resi 81-106 from TRAK1 | Encodes TRAK2 (1-700) C-terminally fused with mScarlet and FKBP. Residues 81-106 corresponding to the dynein regulatory helix are from TRAK1. |
| 248267 | pHR TRAK1 fl mScarlet | Encodes TRAK1 fl C-terminally fused with mScarlet. |
| 248268 | pHR TRAK2 fl mScarlet | Encodes TRAK2 fl C-terminally fused with mScarlet. |
| 248269 | pHR TRAK1 L92A, F95A fl mScarlet | Encodes TRAK1 L92A, F95A fl C-terminally fused with mScarlet. The L92A, F95A mutation disrupts the dynein regulatory helix (DRH). |
| 248270 | pHR TRAK1 L92A, F95A, d373-380 fl mScarlet | Encodes TRAK1 L92A, F95A, d373-380 fl C-terminally fused with mScarlet. |
| 248271 | pHR TRAK2 F92A, M95A fl mScarlet | Encodes TRAK2 F92A, M95A fl C-terminally fused with mScarlet. The F92A, M95A mutation disrupts the dynein regulatory helix (DRH). |
| 248272 | pHR TRAK2 S84A fl mScarlet | Encodes TRAK2 S84A fl C-terminally fused with mScarlet. The S84A mutation prevents S84 phosphorylation. |
| 248273 | pHR TRAK2 S84E fl mScarlet | Encodes TRAK2 S84E fl C-terminally fused with mScarlet. The S84E mutation mimics S84 phosphorylation. |
| 248274 | pHR TRAK2 F92A, M95A, d373-380 fl mScarlet | Encodes TRAK2 F92A, M95A, d373-380 fl C-terminally fused with mScarlet. |
| 248275 | pHR dMiro (1-619) m-sfGFP FLAG I3-01 | Encodes the cytosolic portion of Drosophila Miro (dMiro) C-terminally fused with monomerized sfGFP (V206R), FLAG and the self-assembling I3-01 domain. |
| 248276 | pHR Milton (1-755) mScarlet | Encodes Milton (1-755) C-terminally fused with mScarlet. Expression of this fragment facilitates Milton-mediated motility of dMiro decorated nanocages in human cells. |
| 248277 | pHR FRB m-sfGFP FLAG GB1 I3-01 | Encodes FRB C-terminally fused with monomerized sfGFP (V206R), FLAG and the self-assembling I3-01 domain. |