pHR TRAK1 L92A, F95A (1-702) mScarlet
(Plasmid
#248239)
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PurposeEncodes TRAK1 L92A, F95A (1-702) C-terminally fused with mScarlet. The L92A, F95A mutation disrupts the dynein regulatory helix (DRH).
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRAK1 1-702 mDRH (L92A F95A)
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Alt nameTRAK1
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Alt name10_27
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SpeciesH. sapiens (human)
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MutationL92A, F95A
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Entrez GeneTRAK1 (a.k.a. DEE68, EIEE68, MILT1, OIP106)
- Promoter SFFV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR TRAK1 L92A, F95A (1-702) mScarlet was a gift from Ron Vale (Addgene plasmid # 248239 ; http://n2t.net/addgene:248239 ; RRID:Addgene_248239) -
For your References section:
A molecular switch for coordinating kinesin and dynein transport of mitochondrial cargo. Gladkova C, Paez-Segala MG, Grant WP, Kittisopikul M, Myers SA, Wang Y, Vale RD. Science. 2026 Aug 6;393(6811):601-606. doi: 10.1126/science.aeh1475. Epub 2026 Aug 6. 10.1126/science.aeh1475 PubMed 42561060