pHR Milton (1-755) mScarlet
(Plasmid
#248276)
-
PurposeEncodes Milton (1-755) C-terminally fused with mScarlet. Expression of this fragment facilitates Milton-mediated motility of dMiro decorated nanocages in human cells.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248276 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepHR
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMilton_trunc
-
Alt namemilt
-
Alt name11_34
-
SpeciesD. melanogaster (fly)
-
Entrez Genemilt (a.k.a. Dmel_CG43227, 2L6, CG13777, CG31630, CG43227, Dmel\CG43227, Dmel_CG13777, Dmel_CG31630, Milt, Milton, anon-WO0153538.19, l(2)k04704)
- Promoter SFFV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHR Milton (1-755) mScarlet was a gift from Ron Vale (Addgene plasmid # 248276 ; http://n2t.net/addgene:248276 ; RRID:Addgene_248276) -
For your References section:
A molecular switch for coordinating kinesin and dynein transport of mitochondrial cargo. Gladkova C, Paez-Segala MG, Grant WP, Kittisopikul M, Myers SA, Wang Y, Vale RD. Science. 2026 Aug 6;393(6811):601-606. doi: 10.1126/science.aeh1475. Epub 2026 Aug 6. 10.1126/science.aeh1475 PubMed 42561060