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pHR Miro1 (1-590) m-sfGFP FLAG I3-01
(Plasmid #248233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248233 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Miro1-590 wt -sfGFP-60mer shorter linker
  • Alt name
    RHOT1, I3-01
  • Alt name
    5_31
  • Species
    H. sapiens (human)
  • Entrez Gene
    RHOT1 (a.k.a. ARHT1, MIRO-1, MIRO1)
  • Promoter SFFV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Following lentiviral transduction, 60 copies of the encoded I3-01 fusion self-assemble to form Miro-1 decorated nanocages in cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR Miro1 (1-590) m-sfGFP FLAG I3-01 was a gift from Ron Vale (Addgene plasmid # 248233 ; http://n2t.net/addgene:248233 ; RRID:Addgene_248233)

  • For your References section:

    A molecular switch for coordinating kinesin and dynein transport of mitochondrial cargo. Gladkova C, Paez-Segala MG, Grant WP, Kittisopikul M, Myers SA, Wang Y, Vale RD. Science. 2026 Aug 6;393(6811):601-606. doi: 10.1126/science.aeh1475. Epub 2026 Aug 6. 10.1126/science.aeh1475 PubMed 42561060
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