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pHR TRAK1 L92A, F95A, d373-380 (1-702) mScarlet
(Plasmid #248249)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248249 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRAK1_DRHAla_dSpindly
  • Alt name
    TRAK1
  • Alt name
    11_26
  • Species
    H. sapiens (human)
  • Mutation
    L92A, F95A, d373-380
  • Entrez Gene
    TRAK1 (a.k.a. DEE68, EIEE68, MILT1, OIP106)
  • Promoter SFFV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The L92A, F95A mutation disrupts the dynein regulatory helix (DRH). The Spindly motif 373-380 deletion disrupts dynactin cofactor association.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR TRAK1 L92A, F95A, d373-380 (1-702) mScarlet was a gift from Ron Vale (Addgene plasmid # 248249 ; http://n2t.net/addgene:248249 ; RRID:Addgene_248249)

  • For your References section:

    A molecular switch for coordinating kinesin and dynein transport of mitochondrial cargo. Gladkova C, Paez-Segala MG, Grant WP, Kittisopikul M, Myers SA, Wang Y, Vale RD. Science. 2026 Aug 6;393(6811):601-606. doi: 10.1126/science.aeh1475. Epub 2026 Aug 6. 10.1126/science.aeh1475 PubMed 42561060
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